Flow Cytometry:Article Title: Metalloproteinase inhibitors regulate biliary progenitor cells through sDLK1 in organoid models of liver injury
Article Snippet: .. Primary antibodies Suppliers Dilutions Product number Flow Cytometry CD11b-biotin ebioscience 1:2000 13-0112-82, clone M1/70 CD133-PerCP-efluor710 ebioscience 1:200 46-1331-82, clone 13A4 CD26-FITC BD Biosciences 1:200 559652, clone H194-112 CD31-biotin ebioscience 1:500 13-0311-82, clone 390 CD45.2-biotin ebioscience 1:500 13-0454-82, clone 104 MIC-1-1C3-Dylight650 Novus Biological 1:100 NBP1-18961 Streptavidin-efluor450 ebioscience 1:200 48-4317-82 Immunostaining α-SMA Sigma 1:5000 A2547 Acetylated tubulin Sigma 1:250 T-6793 CD34 Abcam 1:250 81289 CK19 Abcam 1:30 ab15463 CK19 DSHB 1:500 Troma III Desmin Abcam 1:100 15200-1 Epcam Abcam 1:200 ab71916 Hnf4α Santa Cruz 1:100 sc-8987 Hnf4α Santa Cruz 1:100 sc-6556 ki67 (IHC) Thermo Scientific 1:200 RM 9106-S0 ki67 (IF) Biolegend 1:200 652402 Laminin Abcam 1:100 ab11575 Osteopontin R&D 1:500 AF808 Sox9 Millipore 1:600 ab5535 Vimentin Abcam 1:100 ab92547 Donkey anti-Mouse AF488 Jackson ImmunoResearch 1:300 715-545-150 Donkey anti-Rat AF647 Jackson ImmunoResearch 1:300 712-605-153 Donkey anti-Rabbit Cy3 Jackson ImmunoResearch 1:300 711-165-152 Donkey anti-Goat AF488 Jackson ImmunoResearch 1:300 705-545-147 Donkey anti-Goat AF647 Jackson ImmunoResearch 1:300 705-605-003 Western Blots α-SMA Sigma 1:1000 A2547 b-actin-HRP Santa Cruz 1:50,000 sc-47778 cleaved Notch 1 Cell Signaling Technology 1:500 #4147 cleaved Notch 2 Millipore 1:1000 07-1234 DLK1 Abcam 1:1000 ab119930 pERK Cell Signaling Technology 1:1000 #4376 ERK Cell Signaling Technology 1:1000 #9102 PDGF-B Santa Cruz 1:100 sc-7878 PCNA Novocastra 1:500 NCL-PCNA SOX9 Abcam 1:1000 ab76997 pSMAD 2/3 Cell Signaling Technology 1:1000 #3101 SMAD 2/3 Cell Signaling Technology 1:1000 #3102 HRP-conjugated anti Rabbit Cell Signaling Technology 1:10,000 #7074 HRP-conjugated anti Mouse Cell Signaling Technology 1:10,000 #7076 Neutralizing antibody DLK1 (organoid) R&D 2 ng/μl AF8277 3 Supplemental Table 3: Mouse primer sequences used for quantitative RT-PCR. ..
Immunostaining:Article Title: Metalloproteinase inhibitors regulate biliary progenitor cells through sDLK1 in organoid models of liver injury
Article Snippet: .. Primary antibodies Suppliers Dilutions Product number Flow Cytometry CD11b-biotin ebioscience 1:2000 13-0112-82, clone M1/70 CD133-PerCP-efluor710 ebioscience 1:200 46-1331-82, clone 13A4 CD26-FITC BD Biosciences 1:200 559652, clone H194-112 CD31-biotin ebioscience 1:500 13-0311-82, clone 390 CD45.2-biotin ebioscience 1:500 13-0454-82, clone 104 MIC-1-1C3-Dylight650 Novus Biological 1:100 NBP1-18961 Streptavidin-efluor450 ebioscience 1:200 48-4317-82 Immunostaining α-SMA Sigma 1:5000 A2547 Acetylated tubulin Sigma 1:250 T-6793 CD34 Abcam 1:250 81289 CK19 Abcam 1:30 ab15463 CK19 DSHB 1:500 Troma III Desmin Abcam 1:100 15200-1 Epcam Abcam 1:200 ab71916 Hnf4α Santa Cruz 1:100 sc-8987 Hnf4α Santa Cruz 1:100 sc-6556 ki67 (IHC) Thermo Scientific 1:200 RM 9106-S0 ki67 (IF) Biolegend 1:200 652402 Laminin Abcam 1:100 ab11575 Osteopontin R&D 1:500 AF808 Sox9 Millipore 1:600 ab5535 Vimentin Abcam 1:100 ab92547 Donkey anti-Mouse AF488 Jackson ImmunoResearch 1:300 715-545-150 Donkey anti-Rat AF647 Jackson ImmunoResearch 1:300 712-605-153 Donkey anti-Rabbit Cy3 Jackson ImmunoResearch 1:300 711-165-152 Donkey anti-Goat AF488 Jackson ImmunoResearch 1:300 705-545-147 Donkey anti-Goat AF647 Jackson ImmunoResearch 1:300 705-605-003 Western Blots α-SMA Sigma 1:1000 A2547 b-actin-HRP Santa Cruz 1:50,000 sc-47778 cleaved Notch 1 Cell Signaling Technology 1:500 #4147 cleaved Notch 2 Millipore 1:1000 07-1234 DLK1 Abcam 1:1000 ab119930 pERK Cell Signaling Technology 1:1000 #4376 ERK Cell Signaling Technology 1:1000 #9102 PDGF-B Santa Cruz 1:100 sc-7878 PCNA Novocastra 1:500 NCL-PCNA SOX9 Abcam 1:1000 ab76997 pSMAD 2/3 Cell Signaling Technology 1:1000 #3101 SMAD 2/3 Cell Signaling Technology 1:1000 #3102 HRP-conjugated anti Rabbit Cell Signaling Technology 1:10,000 #7074 HRP-conjugated anti Mouse Cell Signaling Technology 1:10,000 #7076 Neutralizing antibody DLK1 (organoid) R&D 2 ng/μl AF8277 3 Supplemental Table 3: Mouse primer sequences used for quantitative RT-PCR. ..
Immunohistochemistry:Article Title: Metalloproteinase inhibitors regulate biliary progenitor cells through sDLK1 in organoid models of liver injury
Article Snippet: .. Primary antibodies Suppliers Dilutions Product number Flow Cytometry CD11b-biotin ebioscience 1:2000 13-0112-82, clone M1/70 CD133-PerCP-efluor710 ebioscience 1:200 46-1331-82, clone 13A4 CD26-FITC BD Biosciences 1:200 559652, clone H194-112 CD31-biotin ebioscience 1:500 13-0311-82, clone 390 CD45.2-biotin ebioscience 1:500 13-0454-82, clone 104 MIC-1-1C3-Dylight650 Novus Biological 1:100 NBP1-18961 Streptavidin-efluor450 ebioscience 1:200 48-4317-82 Immunostaining α-SMA Sigma 1:5000 A2547 Acetylated tubulin Sigma 1:250 T-6793 CD34 Abcam 1:250 81289 CK19 Abcam 1:30 ab15463 CK19 DSHB 1:500 Troma III Desmin Abcam 1:100 15200-1 Epcam Abcam 1:200 ab71916 Hnf4α Santa Cruz 1:100 sc-8987 Hnf4α Santa Cruz 1:100 sc-6556 ki67 (IHC) Thermo Scientific 1:200 RM 9106-S0 ki67 (IF) Biolegend 1:200 652402 Laminin Abcam 1:100 ab11575 Osteopontin R&D 1:500 AF808 Sox9 Millipore 1:600 ab5535 Vimentin Abcam 1:100 ab92547 Donkey anti-Mouse AF488 Jackson ImmunoResearch 1:300 715-545-150 Donkey anti-Rat AF647 Jackson ImmunoResearch 1:300 712-605-153 Donkey anti-Rabbit Cy3 Jackson ImmunoResearch 1:300 711-165-152 Donkey anti-Goat AF488 Jackson ImmunoResearch 1:300 705-545-147 Donkey anti-Goat AF647 Jackson ImmunoResearch 1:300 705-605-003 Western Blots α-SMA Sigma 1:1000 A2547 b-actin-HRP Santa Cruz 1:50,000 sc-47778 cleaved Notch 1 Cell Signaling Technology 1:500 #4147 cleaved Notch 2 Millipore 1:1000 07-1234 DLK1 Abcam 1:1000 ab119930 pERK Cell Signaling Technology 1:1000 #4376 ERK Cell Signaling Technology 1:1000 #9102 PDGF-B Santa Cruz 1:100 sc-7878 PCNA Novocastra 1:500 NCL-PCNA SOX9 Abcam 1:1000 ab76997 pSMAD 2/3 Cell Signaling Technology 1:1000 #3101 SMAD 2/3 Cell Signaling Technology 1:1000 #3102 HRP-conjugated anti Rabbit Cell Signaling Technology 1:10,000 #7074 HRP-conjugated anti Mouse Cell Signaling Technology 1:10,000 #7076 Neutralizing antibody DLK1 (organoid) R&D 2 ng/μl AF8277 3 Supplemental Table 3: Mouse primer sequences used for quantitative RT-PCR. ..
Western Blot:Article Title: Metalloproteinase inhibitors regulate biliary progenitor cells through sDLK1 in organoid models of liver injury
Article Snippet: .. Primary antibodies Suppliers Dilutions Product number Flow Cytometry CD11b-biotin ebioscience 1:2000 13-0112-82, clone M1/70 CD133-PerCP-efluor710 ebioscience 1:200 46-1331-82, clone 13A4 CD26-FITC BD Biosciences 1:200 559652, clone H194-112 CD31-biotin ebioscience 1:500 13-0311-82, clone 390 CD45.2-biotin ebioscience 1:500 13-0454-82, clone 104 MIC-1-1C3-Dylight650 Novus Biological 1:100 NBP1-18961 Streptavidin-efluor450 ebioscience 1:200 48-4317-82 Immunostaining α-SMA Sigma 1:5000 A2547 Acetylated tubulin Sigma 1:250 T-6793 CD34 Abcam 1:250 81289 CK19 Abcam 1:30 ab15463 CK19 DSHB 1:500 Troma III Desmin Abcam 1:100 15200-1 Epcam Abcam 1:200 ab71916 Hnf4α Santa Cruz 1:100 sc-8987 Hnf4α Santa Cruz 1:100 sc-6556 ki67 (IHC) Thermo Scientific 1:200 RM 9106-S0 ki67 (IF) Biolegend 1:200 652402 Laminin Abcam 1:100 ab11575 Osteopontin R&D 1:500 AF808 Sox9 Millipore 1:600 ab5535 Vimentin Abcam 1:100 ab92547 Donkey anti-Mouse AF488 Jackson ImmunoResearch 1:300 715-545-150 Donkey anti-Rat AF647 Jackson ImmunoResearch 1:300 712-605-153 Donkey anti-Rabbit Cy3 Jackson ImmunoResearch 1:300 711-165-152 Donkey anti-Goat AF488 Jackson ImmunoResearch 1:300 705-545-147 Donkey anti-Goat AF647 Jackson ImmunoResearch 1:300 705-605-003 Western Blots α-SMA Sigma 1:1000 A2547 b-actin-HRP Santa Cruz 1:50,000 sc-47778 cleaved Notch 1 Cell Signaling Technology 1:500 #4147 cleaved Notch 2 Millipore 1:1000 07-1234 DLK1 Abcam 1:1000 ab119930 pERK Cell Signaling Technology 1:1000 #4376 ERK Cell Signaling Technology 1:1000 #9102 PDGF-B Santa Cruz 1:100 sc-7878 PCNA Novocastra 1:500 NCL-PCNA SOX9 Abcam 1:1000 ab76997 pSMAD 2/3 Cell Signaling Technology 1:1000 #3101 SMAD 2/3 Cell Signaling Technology 1:1000 #3102 HRP-conjugated anti Rabbit Cell Signaling Technology 1:10,000 #7074 HRP-conjugated anti Mouse Cell Signaling Technology 1:10,000 #7076 Neutralizing antibody DLK1 (organoid) R&D 2 ng/μl AF8277 3 Supplemental Table 3: Mouse primer sequences used for quantitative RT-PCR. ..
Quantitative RT-PCR:Article Title: Metalloproteinase inhibitors regulate biliary progenitor cells through sDLK1 in organoid models of liver injury
Article Snippet: .. Primary antibodies Suppliers Dilutions Product number Flow Cytometry CD11b-biotin ebioscience 1:2000 13-0112-82, clone M1/70 CD133-PerCP-efluor710 ebioscience 1:200 46-1331-82, clone 13A4 CD26-FITC BD Biosciences 1:200 559652, clone H194-112 CD31-biotin ebioscience 1:500 13-0311-82, clone 390 CD45.2-biotin ebioscience 1:500 13-0454-82, clone 104 MIC-1-1C3-Dylight650 Novus Biological 1:100 NBP1-18961 Streptavidin-efluor450 ebioscience 1:200 48-4317-82 Immunostaining α-SMA Sigma 1:5000 A2547 Acetylated tubulin Sigma 1:250 T-6793 CD34 Abcam 1:250 81289 CK19 Abcam 1:30 ab15463 CK19 DSHB 1:500 Troma III Desmin Abcam 1:100 15200-1 Epcam Abcam 1:200 ab71916 Hnf4α Santa Cruz 1:100 sc-8987 Hnf4α Santa Cruz 1:100 sc-6556 ki67 (IHC) Thermo Scientific 1:200 RM 9106-S0 ki67 (IF) Biolegend 1:200 652402 Laminin Abcam 1:100 ab11575 Osteopontin R&D 1:500 AF808 Sox9 Millipore 1:600 ab5535 Vimentin Abcam 1:100 ab92547 Donkey anti-Mouse AF488 Jackson ImmunoResearch 1:300 715-545-150 Donkey anti-Rat AF647 Jackson ImmunoResearch 1:300 712-605-153 Donkey anti-Rabbit Cy3 Jackson ImmunoResearch 1:300 711-165-152 Donkey anti-Goat AF488 Jackson ImmunoResearch 1:300 705-545-147 Donkey anti-Goat AF647 Jackson ImmunoResearch 1:300 705-605-003 Western Blots α-SMA Sigma 1:1000 A2547 b-actin-HRP Santa Cruz 1:50,000 sc-47778 cleaved Notch 1 Cell Signaling Technology 1:500 #4147 cleaved Notch 2 Millipore 1:1000 07-1234 DLK1 Abcam 1:1000 ab119930 pERK Cell Signaling Technology 1:1000 #4376 ERK Cell Signaling Technology 1:1000 #9102 PDGF-B Santa Cruz 1:100 sc-7878 PCNA Novocastra 1:500 NCL-PCNA SOX9 Abcam 1:1000 ab76997 pSMAD 2/3 Cell Signaling Technology 1:1000 #3101 SMAD 2/3 Cell Signaling Technology 1:1000 #3102 HRP-conjugated anti Rabbit Cell Signaling Technology 1:10,000 #7074 HRP-conjugated anti Mouse Cell Signaling Technology 1:10,000 #7076 Neutralizing antibody DLK1 (organoid) R&D 2 ng/μl AF8277 3 Supplemental Table 3: Mouse primer sequences used for quantitative RT-PCR. ..
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